Figure 1
From: Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli
Sequence alignment of the BCO forms expressed in E. coli. fBCO: Protein obtained by subcloning the fBCO-cDNA into pET24b(+) using the NdeI/XhoI restriction sites; mBCO: protein obtained by subcloning the mBCO-cDNA into pET24b(+) using the NdeI/XhoI restriction sites. For comparison, the sequence of the "mature" form of BCO (as determined from the 3-D structure, PDB code 1I19) is reported.