Figure 8
Effective TLR2 inhibition through αT2ib-adenovirus infection in vivo. A-D, Mice were infected 6 d by intravenous (i. v.) or intraperitoneal (i. p.) injection (3 d after thioglycolate injection i. p. was performed) of 1 × 109 pfu of either AdVaT2ib or AdVGFP. A, Splenocytes were isolated from i. v. infected mice and stained with EMA and a CD11b specific antibody. Viable CD11b+ splenocytes were analysed for EGFP expression by flow cytometry. As control splenocytes from non-infected mice were applied. B, Peritoneal wash out cells were drawn 6 d upon adenoviral infection as indicated or from non-infected mice (control). Cells that were adherent after 2 h of cell culturing were stained with EMA and analysed for EGFP expression. C, Peritoneal macrophages from mice infected with adenoviruses i. p. (white column, AdVGFP; black column, AdVaT2ib) or from a non-infected TLR2-/- mouse (grey column) were challenged as indicated (none, non challenged) for 24 h after which supernatants were sampled for cytokine content by ELISA (one result of two similar results out of two independent experiments, n = 2 per group). D, Splenocytes from mice infected i. v. (white column, AdVGFP; black column, AdVaT2ib) were challenged for 24 h upon which supernatant cytokine concentration was determined by ELISA (representative result of one out of two independent experiments, n = 4 per group; n.d., not detected).