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Table 1 List of different regulatory elements used to design a set of gag piggyBac vector constructs.

From: Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production

Construct Enhancer Promoter Intron upstream Gene Intron downstream PolyA Final pXLBacII construct
pHSP70   Drosophila hsp70   gag   SV40sti/polyA pXLHSP70Gag
pIE1 hr5 Baculovirus AcMNPV ie1   gag   (Heliothis) hel2polyA pXLIE1Gag
pIE1 hr5 Baculovirus AcMNPV ie1   Neo   hel2polyA pXLNeo
pIE1-SV hr5 Baculovirus AcMNPV ie1   gag   SV40sti/polyA pXLIE-SVGag
pActin   Drosophila actin 5C   gag   hel2-polyA pXLActinGag
pActin-SV   Drosophila actin 5C   gag   SV40sti/polyA pXLActin-SVGag
409-FOR hr5 Baculovirus AcMNPV ie1 + gag   hel2polyA pXL409Gag
410-FOR hr5 Baculovirus AcMNPV ie1   gag + hel2polyA pXL410Gag
411-FOR   Drosophila actin 5C + gag   hel2-polyA pXL411Gag
412-FOR   Drosophila actin 5C   gag + hel2-polyA pXL412Gag
Hr3ieLuc hr3 Baculovirus AcMNPV ie1   gag    pXlHr3ieGag
pBSII-IE1-orf   Baculovirus AcMNPV ie1   gag   pBSII-IE1-orf polyA pXLBSII-IE1Gag
  1. The promoters tested included Drosophila hsp70, AcMNPV ie1 and Drosophila actin 5C [30, 31] (column 3). The promoter enhancers used were hr5 [31] and hr3 [30] (column 2). Poly-adenylation (polyA) sequences included SV40sti/polyA, hel2polyA [31] and a polyA tail isolated from the pBSII-IE1-orf helper plasmid [14, 15] (column 7). 409-FOR and 410-FOR are pIE1 vectors containing a 133 bp intron [32] (column 4 and 6) upstream or downstream of the gag gene, respectively. 411-FOR and 412-FOR are pActin vectors containing the intron upstream or downstream of the gag gene, respectively. Briefly, different promoters, enhancers, polyA sequences or the intron were removed from the construct described in column 1 and coupled to gag in the transposable pXLBacII vector [14, 15] (column 8).