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Figure 4 | BMC Biotechnology

Figure 4

From: Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production

Figure 4

Western blot immunodetection of PB and BV VLPs. A: Anti-p24 western blot. Lane 1-1.2 ng Pr41 std. Lane 2-2.4 ng Pr41 std. Lane 3-4.8 ng Pr41 std. Lane 4-7.2 ng Pr41 std. Lane 5- purified PB VLPs from the 20/30% Optiprep® interphase (22 ug of total soluble protein loaded). Lane 6- purified BV VLPs from the 20/30% Optiprep® interphase (0.68 ug of total soluble protein loaded). B: Anti-gp64 western blot. Lane 1- Supernatant from cells infected with negative baculovirus (no gag insert). Lane 2- purified PB VLPs from the 10/20% Optiprep® interphase. Lane 3- purified PB VLPs from the 20/30% Optiprep® interphase. Lane 4- purified BV VLPs from the 20/30% Optiprep® interphase. The respective sizes from markers run on the same gels are indicated in kDA on the left of both blots.

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BMC Biotechnology

ISSN: 1472-6750

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