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Figure 2 | BMC Biotechnology

Figure 2

From: Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production

Figure 2

RCA amplification carried out on DNA extracted from NeoGag expressing transgenic cells. The RCA product was digested with Mlu1 and visualised on an 0.8% agarose gel. Lane 1- Fermentas Bioline hyperladder 1 with sizes indicated in basepairs. Lane 2- positive control (pXLBacIINeoGag). Lane 3- Sf21 NeoGag genomic DNA. Lane 4- Sf21 NeoGag genomic DNA spiked with 7 ng of the positive control. Lane 5- Sf21 NeoGag genomic DNA spiked with 700 pg of positive control. Lane 6- Sf21 NeoGag genomic DNA spiked with 70 pg of positive control. Lane 7- Sf21 NeoGag genomic DNA spiked with 7 pg of positive control.

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