Figure 2From: Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP productionRCA amplification carried out on DNA extracted from NeoGag expressing transgenic cells. The RCA product was digested with Mlu1 and visualised on an 0.8% agarose gel. Lane 1- Fermentas Bioline hyperladder 1 with sizes indicated in basepairs. Lane 2- positive control (pXLBacIINeoGag). Lane 3- Sf21 NeoGag genomic DNA. Lane 4- Sf21 NeoGag genomic DNA spiked with 7 ng of the positive control. Lane 5- Sf21 NeoGag genomic DNA spiked with 700 pg of positive control. Lane 6- Sf21 NeoGag genomic DNA spiked with 70 pg of positive control. Lane 7- Sf21 NeoGag genomic DNA spiked with 7 pg of positive control.Back to article page