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Figure 3 | BMC Biotechnology

Figure 3

From: Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy

Figure 3

Real-time monitoring of circadian molecular oscillations by PMT in rat-1, MEF, FBHE and rat astrocytes. Photomultiplier-tube (PMT)-based bioluminescence was monitored in Dbp:luc-pT2A and Bmal1:luc-pT2A stably transfected cells. (A) Rat-1 cells stably transfected with Dbp:luc-pT2A showing circadian bioluminescence oscillations. A representative example of 12 samples with similar results. (B) MEFs stably transfected with Dbp:luc-pT2A showing circadian bioluminescence oscillations. A representative example of 9 samples with similar results. Note the similar phases of circadian oscillations in rat-1 and MEFs, although the cycle duration is slightly different in the two cells. (C) Fetal bovine heart endothelial (FBHE) cells stably transfected with Bmal1:luc-pT2A showing circadian bioluminescence oscillations. A representative example of 13 samples with similar results. (D) Rat astrocytes stably transfected with Bmal1:luc-pT2A showing circadian bioluminescence oscillations. A representative example of 4 samples with similar results. (E) Bmal1:luc-pT2A driven bioluminescence in FBHE cells exhibits almost anti-phasic rhythms relative to the Dbp:luc-pT2A driven bioluminescence rhythms shown in MEF cells. Peak phases of Dbp:luc-pT2A stably transfected MEFs (n = 9) and Bmal1:luc-pT2A stably transfected FBHE cells (n = 13). Mean phase ± SD is indicated to the right of the figure.

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