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Figure 2 | BMC Biotechnology

Figure 2

From: Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy

Figure 2

Tol2 transposon-based vector system can generate stably transfected cells with extremely high efficiency. (A) Colony formation assay examining the efficiency generating Dbp:luc-pT2A stably transfected rat-1 cells. Dish 1: negative control condition without co-transfection of transposase expression plasmid (pCAGGS-TP). Dish 2: transfection condition of 1 μg of Dbp:luc-pT2A plasmid and 0.2 μg of pCAGGS-TP plasmid. Dish 3: transfection condition of 1 μg of Dbp:luc-pT2A plasmid and 1 μg of pCAGGS-TP plasmid. The cells were fixed and stained after two weeks selection culture. (B) Quantitative data of the dishes shown in (A). (C) Tol2 transposon vector system allows the generation of Dbp:luc-pT2A stably transfected cells with over 20-fold higher efficiency compared with the system lacking co-transfection of pGAGGS-TP plasmid. Data are mean ± SEM numbers of cell colonies. Hash indicates P < 0.005, Asterisk indicates P < 0.05, t-test (n = 3)

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BMC Biotechnology

ISSN: 1472-6750

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