Comparative analysis of right element mutant lox sites on recombination efficiency in embryonic stem cells

BMC Biotechnology

Table 1 Frequency of site-specific integration

Cell line	lox66	loxJTZ17	loxKR1	loxKR2	loxKR3	loxKR4
Bs2	13.4 ± 4.3	18.0 ± 5.4	13.9 ± 2.5	19.2 ± 6.9	18.0 ± 2.4	17.5 ± 1.2
Bs17	11.3 ± 0.6	17.2 ± 2.2	13.3 ± 1.5	22.7 ± 3.3	17.3 ± 2.4	17.5 ± 3.7
Bs19	14.2 ± 2.9	13.3 ± 3.1	10.5 ± 1.8	18.0 ± 5.7	11.9 ± 3.4	12.9 ± 1.1
Bs21	13.8 ± 1.3	15.8 ± 2.6	14.4 ± 2.3	18.9 ± 3.6	13.9 ± 4.5	16.9 ± 2.7

Twenty micrograms of the integration plasmid and 10 μg of the Cre-expressing vector were coelectroporated. After drug selection for 7 days, colonies were stained with X-gal, and the percentage of positive colonies was scored as the frequency of site-specific integration. The means ± SD of three independent electroporations are represented.

ISSN: 1472-6750