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Table 1 Frequency of site-specific integration

From: Comparative analysis of right element mutant lox sites on recombination efficiency in embryonic stem cells

Cell line lox66 loxJTZ17 loxKR1 loxKR2 loxKR3 loxKR4
Bs2 13.4 ± 4.3 18.0 ± 5.4 13.9 ± 2.5 19.2 ± 6.9 18.0 ± 2.4 17.5 ± 1.2
Bs17 11.3 ± 0.6 17.2 ± 2.2 13.3 ± 1.5 22.7 ± 3.3 17.3 ± 2.4 17.5 ± 3.7
Bs19 14.2 ± 2.9 13.3 ± 3.1 10.5 ± 1.8 18.0 ± 5.7 11.9 ± 3.4 12.9 ± 1.1
Bs21 13.8 ± 1.3 15.8 ± 2.6 14.4 ± 2.3 18.9 ± 3.6 13.9 ± 4.5 16.9 ± 2.7
  1. Twenty micrograms of the integration plasmid and 10 μg of the Cre-expressing vector were coelectroporated. After drug selection for 7 days, colonies were stained with X-gal, and the percentage of positive colonies was scored as the frequency of site-specific integration. The means ± SD of three independent electroporations are represented.