|
Cell line
|
lox66
|
loxJTZ17
|
loxKR1
|
loxKR2
|
loxKR3
|
loxKR4
|
|---|
|
Bs2
|
13.4 ± 4.3
|
18.0 ± 5.4
|
13.9 ± 2.5
|
19.2 ± 6.9
|
18.0 ± 2.4
|
17.5 ± 1.2
|
|
Bs17
|
11.3 ± 0.6
|
17.2 ± 2.2
|
13.3 ± 1.5
|
22.7 ± 3.3
|
17.3 ± 2.4
|
17.5 ± 3.7
|
|
Bs19
|
14.2 ± 2.9
|
13.3 ± 3.1
|
10.5 ± 1.8
|
18.0 ± 5.7
|
11.9 ± 3.4
|
12.9 ± 1.1
|
|
Bs21
|
13.8 ± 1.3
|
15.8 ± 2.6
|
14.4 ± 2.3
|
18.9 ± 3.6
|
13.9 ± 4.5
|
16.9 ± 2.7
|
- Twenty micrograms of the integration plasmid and 10 μg of the Cre-expressing vector were coelectroporated. After drug selection for 7 days, colonies were stained with X-gal, and the percentage of positive colonies was scored as the frequency of site-specific integration. The means ± SD of three independent electroporations are represented.
