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Figure 2 | BMC Biotechnology

Figure 2

From: Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host

Figure 2

Copy-number induction in MW005. Panel A. Aliquots (5 μl) of fUL#SB28 (gel i), WRM0636aA04 (gel ii) or WRM067aC01 (gel iii) pCC1FOS-based DNA, isolated from equivalent numbers of cells from either non-induced (NI) or copy-number-induced (I) cultures of EPI300 or MW005 and incubated with either BamHI (gel i) or NcoI (gels ii & iii), were electrophoresed through a 0.7% (w/v) agarose gel either undiluted (lanes 1, 8) or after 2- (lanes 2, 9), 4- (lanes 3, 10), 8- (lanes 4, 11), 16- (lanes 5, 12), 32- (lanes 6, 13) or 64-fold (lanes 7, 14) dilution. Panel B. Aliquots (5 μl) of A02_CBP0333 (gel i), A10_CBP1191 (gel ii) or H12_CBP0642 (gel iii) pCC1BAC-based DNA, isolated from equivalent numbers of cells from either non-induced (NI) or copy-number-induced (I) cultures of EPI300 or MW005 and incubated with NcoI, were electrophoresed through a 0.7% (w/v) agarose gel either undiluted (lanes 1, 7) or after 2- (lanes 2, 8), 4- (lanes 3, 9), 8- (lanes 4, 10), 16- (lanes 5, 11) or 32-fold (lanes 6, 12) dilution. M = DNA ladder (kb).

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BMC Biotechnology

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