Table 3 Sequences of used promoters and primers
From: Promoter knock-in: a novel rational method for the fine tuning of genes
Primer | Sequence |
|---|---|
Fw-EcoRI-p37 | gggggaattccttacatgaaaaaggttcttg |
Rv-BamHI-p37 | ttttggatcccatctttgtttcctccgagaaaaatgacatataccacatgg |
Fw-EcoRI-p55 | gggggaattccttagaaggaatttgttcttg |
Rv-BamHI-p55 | ttttggatcccatctttgtttcctccgagatacctaaaaattatacc |
Fw-EcoRI-P1 | ttttgaattcgtgtaggctggagctgcttc |
Rv-HindIII-P2 | ggggaagcttcatatgaatatcctccttag |
Fw-ppc-HIS-RBS-37 | acattactacgcaatgcggaatattgttcgttgtggtgatggtgatggtgcgccatctttgtttcctccgagaaaaatgac |
Fw-ppc-HIS-RBS-55 | acattactacgcaatgcggaatattgttcgttgtggtgatggtgatggtgcgccatctttgtttcctccgagatacctaa |
Rv-ppc-P2 | cgtgaaggatacagggctatcaaacgataagatggggtgtctggggtaatcatatgaatatcctccttag |
Rv-ppc-3-P2 | atcaagcccacccgcgaactgataacccaggtaattcaccatttttgctggcattaacatatgaatatcctccttag |
Fw-ppc-37 | tccttcacgtcgcattggcgcgaatatgctcgggctttgcttttcgtcgtcaaaaatgacatataccacatgga |
Fw-ppc-55 | tttgccgagcatactgacattactacgcaatgcggaatattgttcgttcatctttgtttcctccgagatacctaaaaattataccacatcaac |