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Table 2 Expression of ppc gene with the artificial promoters relative to the natural promoter in strain MG1655 (wt) and MG1655 ΔackA-pta, ΔpoxB (3KO).

From: Promoter knock-in: a novel rational method for the fine tuning of genes

Strain Flask-scale Bioreactor-scale
  Expression of ppcrelative to WT Expression of ppcrelative to WT
  mRNA (2-ΔΔct) PEP carboxylase activity mRNA (2-ΔΔct) PEP carboxylase activity
wt 1.0
(0.9-1.1)
1.00 ± 0.002 1.0
(0.9-1.1)
1.00 ± 0.002
3KO 0.7
(0.5-0.9)
0.82 ± 0.003 0.8
(0.7-0.9)
0.92 ± 0.005
wt - p37 3.9
(3.3-4.5)
3.33 ± 0.015 6.7
(5.8-7.8)
5.24 ± 0.013
wt - p55 2.5
(2.1-3.0)
1.79 ± 0.002 5.0
(4.7-5.2)
3.22 ± 0.014
3KO - p37 3.7
(3.3-4.3)
3.56 ± 0.002 5.7
(5.3-6.2)
4.71 ± 0.019
3KO - p55 2.3
(2.0-2.7)
2.02 ± 0.016 4.2
(3.9-4.5)
2.55 ± 0.007