Table 2 Expression of ppc gene with the artificial promoters relative to the natural promoter in strain MG1655 (wt) and MG1655 ΔackA-pta, ΔpoxB (3KO).
From: Promoter knock-in: a novel rational method for the fine tuning of genes
Strain | Flask-scale | Bioreactor-scale | ||
|---|---|---|---|---|
| Â | Expression of ppcrelative to WT | Expression of ppcrelative to WT | ||
|  | mRNA (2-ΔΔct) | PEP carboxylase activity | mRNA (2-ΔΔct) | PEP carboxylase activity |
wt | 1.0 (0.9-1.1) | 1.00 ± 0.002 | 1.0 (0.9-1.1) | 1.00 ± 0.002 |
3KO | 0.7 (0.5-0.9) | 0.82 ± 0.003 | 0.8 (0.7-0.9) | 0.92 ± 0.005 |
wt - p37 | 3.9 (3.3-4.5) | 3.33 ± 0.015 | 6.7 (5.8-7.8) | 5.24 ± 0.013 |
wt - p55 | 2.5 (2.1-3.0) | 1.79 ± 0.002 | 5.0 (4.7-5.2) | 3.22 ± 0.014 |
3KO - p37 | 3.7 (3.3-4.3) | 3.56 ± 0.002 | 5.7 (5.3-6.2) | 4.71 ± 0.019 |
3KO - p55 | 2.3 (2.0-2.7) | 2.02 ± 0.016 | 4.2 (3.9-4.5) | 2.55 ± 0.007 |