Table 1 Expression of the ppc gene with the artificial p37 promoter inserted with the 3 different strategies at transcriptomic level (qPCR) and enzyme expression level.
From: Promoter knock-in: a novel rational method for the fine tuning of genes
| Â | Strategy I | Strategy II | Strategy III | |||
|---|---|---|---|---|---|---|
|  | mRNA (2-ΔΔct) | PEP carboxylase activity | mRNA (2-ΔΔct) | PEP carboxylase activity | mRNA (2-ΔΔct) | PEP carboxylase activity |
Wild-type | 1.0 (0.8-1.3) | 1.00 ± 0.002 | 1.0 (0.7-1.5) | 1.00 ± 0.002 | 1.0 (0.9-1.2) | 1.00 ± 0.002 |
mutant | 0.4 (0.3-0.5) | 0.50 ± 0.003 | 0.2 (0.1-0.3) | 0.13 ± 0.001 | 3.9 (3.3-4.5) | 3.32 ± 0.015 |