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Table 1 Expression of the ppc gene with the artificial p37 promoter inserted with the 3 different strategies at transcriptomic level (qPCR) and enzyme expression level.

From: Promoter knock-in: a novel rational method for the fine tuning of genes

  Strategy I Strategy II Strategy III
  mRNA
(2-ΔΔct)
PEP carboxylase activity mRNA
(2-ΔΔct)
PEP carboxylase activity mRNA
(2-ΔΔct)
PEP carboxylase activity
Wild-type 1.0
(0.8-1.3)
1.00 ± 0.002 1.0
(0.7-1.5)
1.00 ± 0.002 1.0
(0.9-1.2)
1.00 ± 0.002
mutant 0.4
(0.3-0.5)
0.50 ± 0.003 0.2
(0.1-0.3)
0.13 ± 0.001 3.9
(3.3-4.5)
3.32 ± 0.015