Figure 2
From: Promoter knock-in: a novel rational method for the fine tuning of genes
Strategies to replace the natural ppc promoter by an artificial promoter. (a) Strategy I: insertion of a promoter + artificial RBS and a polyHis-tag sequence between the ppc gene and its natural promoter (b) Strategy II: replacement of natural promoter by a promoter + artificial RBS and a polyHis-tag sequence (c) Strategy III: replacement of natural promoter by artificial promoter, keeping the natural RBS.