TY - JOUR AU - Kuystermans, Darrin AU - Dunn, Michael J. AU - Al-Rubeai, Mohamed PY - 2010 DA - 2010/03/22 TI - A proteomic study of cMyc improvement of CHO culture JO - BMC Biotechnology SP - 25 VL - 10 IS - 1 AB - The biopharmaceutical industry requires cell lines to have an optimal proliferation rate and a high integral viable cell number resulting in a maximum volumetric recombinant protein product titre. Nutrient feeding has been shown to boost cell number and productivity in fed-batch culture, but cell line engineering is another route one may take to increase these parameters in the bioreactor. The use of CHO-K1 cells with a c-myc plasmid allowing for over-expressing c-Myc (designated cMycCHO) gives a higher integral viable cell number. In this study the differential protein expression in cMycCHO is investigated using two-dimensional gel electrophoresis (2-DE) followed by image analysis to determine the extent of the effect c-Myc has on the cell and the proteins involved to give the new phenotype. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-10-25 DO - 10.1186/1472-6750-10-25 ID - Kuystermans2010 ER -