Inhibition of Aβ production in cultured cells by fragments of Gleevec. A. Structures of Gleevec and its fragments used for the treatment of cultured cells and zebrafish embryos. Four fragments of Gleevec are named as AD28, AD94, AD95 and AD115. B and C. Inhibition of γ-secretase cleavage of APP was determined by measuring the levels of Aβ40 (B) and Aβ42 (C) in the conditioned-media by ELISA. Levels of Aβ from cells treated with 0.1% of DMSO were normalized to 1 and used to calculate the relative levels of Aβ from cells treated with DAPT, Gleevec, and its fragments. Standard errors of means are illustrated by bars.