Identification of salt stress conditions for wild-type S. cerevisiae (BY4741). Sectors in plates marked with red dot are wild-type, while other sectors represent salt hypersensitive yeast mutants obtained through random UV mutagenesis. Wild-type yeast BY4741 shows salt sensitivity from 500 mM NaCl, with complete growth arrest at 2.0 M NaCl. In contrast, salt hypersensitive mutants shs-2, shs-4, shs-6 and shs-8 (isolated in the BY4741 background) show growth retardation from 500 mM. Similar amount of inoculums were used in all the plates.