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Figure 3 | BMC Biotechnology

Figure 3

From: Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring

Figure 3

Influence of different initial biomass concentrations on growth and product formation of E. coli BL21(DE3) pRhotHi-2-EcFbFP. A) Scattered light intensity (mean of eight parallel wells per condition). B) EcFbFP fluorescence intensity (mean of eight parallel wells per condition). Vertical dashed line: time of induction with 0.1 mM IPTG after 4.9 h. Experimental conditions: MDG medium, filling volume per well 200 μL, shaking frequency 950 rpm, shaking diameter 3 mm, temperature 37°C, measurement with BioLector.

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