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Figure 2 | BMC Biotechnology

Figure 2

From: Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring

Figure 2

Influence of different replication tools on growth of 96 cultures of E. coli PR02. Replication tools: A) 8-channel pipette, thawed and mixed cryoculture. B) Disposable plastic replicator, thawed and mixed cryoculture. C) Steel replicator, fixed pins, thawed and mixed cryoculture. D) Steel replicator, fixed pins, thawed cryoculture, not mixed. E) Steel replicator, fixed pins, frozen cryoculture. F) Steel replicator, spring-loaded pins, frozen cryoculture. Experimental conditions: TB medium with 5 g/L glycerol, filling volume per well 200 μL, shaking frequency 950 rpm, shaking diameter 3 mm, temperature 37°C, measurement with BioLector.

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BMC Biotechnology

ISSN: 1472-6750

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