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Figure 1 | BMC Biotechnology

Figure 1

From: Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring

Figure 1

Scheme of a typical high-throughput cultivation process. After transformation, the clones are plated and grown on agar. Colonies are transferred with sterile toothpicks to a preculture-MTP and cultivated. Glycerol is added and the MTP is frozen (cryoculture). After thawing and mixing, liquid is transferred from the cryoculture-MTP to a new preculture-MTP. This MTP is incubated and subsequently a new main culture-MTP is inoculated with liquid from the preculture-MTP. At a defined point of time, inducer is added to the main culture to induce protein expression.

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BMC Biotechnology

ISSN: 1472-6750

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