Scheme of a typical high-throughput cultivation process. After transformation, the clones are plated and grown on agar. Colonies are transferred with sterile toothpicks to a preculture-MTP and cultivated. Glycerol is added and the MTP is frozen (cryoculture). After thawing and mixing, liquid is transferred from the cryoculture-MTP to a new preculture-MTP. This MTP is incubated and subsequently a new main culture-MTP is inoculated with liquid from the preculture-MTP. At a defined point of time, inducer is added to the main culture to induce protein expression.