Table 1 Oligonucleotides used in this work
From: A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
Oligonucleotide | Sequence (5'-3') |
|---|---|
Cre1YILY-1UF | ATTGGTTTCdUTGGTGTATATACTGTATGGTGGTGCAGCTATGCACATAGTAAAAGTCGAAGATGATTT |
Cre1YILY-1UR | AGAAACCAAdUCGCAAAGAACAATGG |
Cre1YILY-1XF | PO4TGGTGTATATACTGTATGGTGGTGCAGCTATGCACATAGTAAAAGTCGAAGATGATTT |
Cre1YILY-1XR | PO4-AGAAACCAATCGCAAAGAACAATGG |
Pfu-V93Q-F | GTGGAAACTTTATTTGGAACATCCCCAAGATCAGCCCACTATTAGAGAAAAAGT |
Pfu-V93Q-R | ACTTTTTCTCTAATAGTGGGCTGATCTTGGGGATGTTCCAAATAAAGTTTCCAC |
Pfu-upstream-F | GCGTTACTGCCTGGGGAAGA |
Pfu-R | ACCGCCGGdUACCGGATTTTTTAATGTTAAGCCAGGAAG |
Pfu-F | AGCAGAAAAAGdUAGCAATTCGTCGTGCATCTGTTTG |
Pfu-downstream-R | GGCCCCAAGGGGTTATGCTA |
Sso7d-1F | ACCGGCGGdUGGCGGTGCAACCGTAAAGTTCAAGTACAAAGGCGAAGAAAAAGAGGTAGACATCTCCAAGATCAAGAAAGT |
Sso7d-1R | ACTTTCTTGAdUCTTGGAGATGTCTACCTCTTTTTCTTCGCCTTTGTACTTGAACTTTACGGTTGCACCGCCACCGCCGGT |
Sso7d-2F | ATCAAGAAAGdUATGGCGTGTGGGCAAGATGATCTCCTTCACCTACGACGAGGGCGGTGGCAAGACCGGCCGT |
Sso7d-2R | ACGGCCGGTCdUTGCCACCGCCCTCGTCGTAGGTGAAGGAGATCATCTTGCCCACACGCCATACTTTCTTGAT |
Sso7d-3F | AGACCGGCCGdUGGTGCGGTAAGCGAAAAGGACGCGCCGAAGGAGCTGCTGCAGATGCTGGAGAAGCAGAAAAAGT |
Sso7d-3R | ACTTTTTCTGCdUTCTCCAGCATCTGCAGCAGCTCCTTCGGCGCGTCCTTTTCGCTTACCGCACCACGGCCGGTCT |
GFP-Y40STOP-F | GGGTGAAGGTGATGCTACATAAGGAAAGCTTACC |
GFP-Y40STOP-R | GGTAAGCTTTCCTTATGTAGCATCACCTTCACCC |