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Figure 1 | BMC Biotechnology

Figure 1

From: A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

Figure 1

Principle of uracil-excision based DNA fusion. In uracil-excision based DNA fusions, dU nucleotides replace selected thymidine nucleotides in DNA and is subsequently removed by e.g. the USERâ„¢ enzyme, leaving the upstream nucleotide sequence unstable. When the resulting, compatible single stranded overhangs are combined they can be transformed directly into bacteria without prior ligation to yield a stable recombinant molecule.

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