Figure 3

Neovascularization on melanoma tumor was impaired by endostatin. (A and B) - Immunohistochemical analyses of the melanoma tumor microenvironment, after 7 days of treatment, showing the differences found in the vascular structure in the (A) control mice treated with LM(pSecTag) cells and (B) mice treated with LM(pSecTag-end) cells. The neovascularization was quantitatively analyzed in the tumor tissue immunohistochemistry stained using the CD34 antibody (C) Number of vascular structures (n = 5) (p = 0.008) ; (D) Number of functional vasculature (vessel with erythrocyte inside) (n = 5) (p = 0.047); and (E) vascular area (n = 5) (p = 0.2). Mean +/- SD.