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Table 1 CFUs of recombinant strains on YEPD and ura- selective media.

From: Heterologous expression of a tannic acid-inducible laccase3 of Cryphonectria parasitica in Saccharomyces cerevisiae

Strain

CFUs/plate1

 

YEPD2

ura-

Mock transformant

  

   YEPD plating

917 ± 27 3

892 ± 22

   ura- plating

819 ± 16

813 ± 24

TYEGLAC3-1

  

   YEPD plating

302 ± 17

189 ± 6

   ura- plating

98 ± 5

100 ± 4

TYEGLAC3-5

  

   YEPD plating

311 ± 14

217 ± 8

   ura- plating

103 ± 3

112 ± 4

TYEGLAC3-7

  

   YEPD plating

351 ± 11

218 ± 7

   ura- plating

112 ± 5

112 ± 3

  1. 1 Colony-forming units per plate
  2. 2 For each strain, 1 × 107 cells were inoculated into corresponding media, as described in Materials and methods.
  3. 3 Five replicates of each strain were used, and each experiment was repeated three times. Values are shown as the means ± standard deviation.