Figure 5

Schematic diagram of the yeast expression vector for laccase3. The boxes represent genes or their corresponding functional domains. (A) Schematic diagram of the fusion construct cloned into the pYEGPD-TER plasmid and the sequence covering the links of the GPD promoter-rice Amy1A signal peptides-LAC3. (B) Schematic diagram of the fusion construct cloned into the pYEGAL-TER plasmid and the sequence covering the links of the GAL promoter-rice Amy1A signal peptides-LAC3. The translation start codon and the first codon of LAC3 are in bold. pGPD, promoter of glyceraldehyde-3-phosphate dehydrogenase; pGAL1, promoter of galactokinase; ASP, rice amylase1A signal peptides; mLAC3, mature peptide of laccase3; tGAL7, terminator of galactose-1-phosphate uridylyltransferase.