Figure 2

Western blot analysis and native PAGE activity staining. Culture supernatant 2 days after cultivation was resolved on native PAGE followed by activity staining using the substrate solution (A). Coomassie staining (B) and Western blot analysis of the twin gel (C) were performed. Lanes 1 and 2 contain 1 and 5 mU laccase, respectively, from Trametes versicolor as controls. Lane 3 contains 12 μl 40× concentrated 3-day-old culture supernatant of a mock transformant. Lanes 4 and 5 contain 12 μl of non-concentrated and 40× concentrated, respectively, 3-day-old culture supernatant of TYEGLAC3-1.