PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome

Table 3 PCR analysis of MC₁ plants

MC₁-Parent line	Plants tested	Positive for target locus ^a	Positive for recombinase gene ^b	Positive for excision and recombinase gene ^c	Positive for excision and negative for unexcised product ^d
phiC31.22.3	11	10	11	8	8
phiC31.22.15	34	31	32	23	11
phiC31.31.40	17	16	16	8	8
phiC31.31.83	17	17	17	17	17
phiC31.34.9	68	44	23	15	15
phiC31.34.20	44	33	40	22	19

^aPrimers eand fyielded the 1.26 and/or 0.44 kb target fragment.
^bPrimers gand hfailed to detect the 0.71 kb phiC31 fragment.
^cPrimers eand fyielded the 0.44 kb excision fragment and primers gand hdetect the 0.71 kb phiC31 fragment.
^dPrimers eand fyielded the 0.44 kb excision fragment and failed to detect a 1.26 kb target fragment.

ISSN: 1472-6750