PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome

Table 2 PCR analysis of BC₁ and S₁ plants

TR₁-Parent line	Plants tested	Positive for target locus ^a	Positive for excision ^b	Positive for excision and negative for recombinase gene ^c	Positive for recombinase gene and negative for target locus ^d
phiC31.22.3	42	23	23	21	0
phiC31.22.15	17	16	16	2	0
phiC31.22.23	68	29	21	3	1
phiC31.22.29	61	44	44	24	1
phiC31.22.87	6	3	3	1	1
phiC31.31.1	59	55	28	14	1
phiC31.31.13	44	29	22	10	14
phiC31.31.23	14	8	8	2	4
phiC31.31.29	62	59	59	22	0
phiC31.31.36	48	27	25	13	5
phiC31.34.2	43	18	18	1	0
phiC31.34.5	44	23	8	0	2
phiC31.34.9	44	13	13	0	9
phiC31.34.20	42	26	26	0	1
phiC31.34.24	41	38	38	0	0

^aPrimers eand fyielded the 1.26 and/or 0.44 kb target fragment.
^bPrimers eand fyielded the 0.44 kb excision fragment.
^cPrimers eand fyielded the 0.44 kb excision fragment while primers gand hfailed to detect the 0.71 kb phiC31 fragment.
^dPrimers gand hyielded the 0.71 kb phiC31 fragment while primers eand ffailed to detect the 1.26 and/or 0.44 kb target fragment.

ISSN: 1472-6750