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Table 1 PCR analysis of TR1 plants

From: PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome

TA Parent line

Plants tested

Positive for recombinase gene aand target locus b

Positive for excision c

Positive for excision and negative for unexcised product d

phiC31.22

88

65

47

35

phiC31.31

67

31

21

14

phiC31.34

43

19

17

2

  1. aPrimers gand hyielded the 0.71 kb phiC31 fragment.
  2. bPrimers eand fyielded the 1.26 and/or 0.44 kb fragment.
  3. cPrimers eand fyielded the 0.44 kb excision fragment.
  4. dPrimers eand ffailed to detect a 1.26 kb target fragment.