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Figure 6 | BMC Biotechnology

Figure 6

From: PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome

Figure 6

PCR analysis for site-specific recombination and the presence of the phiC31 gene in the MC 1 generation. a) PCR reactions with primers eand f(Fig. 1) or b) with primers gand h(Fig. 1) on manually crossed lines; (lanes 1, 2) MC1-phiC31.22.3, 22.15; (lanes 3, 4) MC1-phiC31.31.40, 31.83; (lane 5, 6) MC1-phiC31.34.9, 34.20. Control lanes are B (blank, no DNA); E (excision, pN3-phiC31exc); N (no excision, pN3-phiC31); P (recombinase, pCOXS3-phiC31).

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