Figure 5
S 1 plants examined by Southern blot analysis for excision and segregation of phiC31 gene. a) Genomic DNA cleaved with EcoRV hybridized with a 32P-labeled GUS1350 probe (Fig. 1). b) Genomic DNA digested with XhoI and hybridized with a 32P-labeled NPT690 probe. Plant lines (lanes 1, 2) S1-phiC31.22.3.18.1, 22.29.7.1; (lanes 3, 4) S1- phiC31.31.31.1.1, 31.31.13.1; (lane 5, 6) S1-phiC31.34.2.10.1; 34.9.20.1. Control lanes are wt (wild type Arabidopsis genomic DNA), TA3-phiC31.22, (target lines), TR1- phiC31.22.23 (phiC31 recombinase expression line).