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Table 2 Detailed overview of characterized antibodies

From: Immunohistochemical detection of transgene expression in the brain using small epitope tags

Epitope Antibody Clonality Company Product N° Concentration Optimal dilution for IHC based on current study Optimal concentration (μg/ml) for IHC based on current study
3flag and flag Anti-flag polyclonal1 Polyclonal Sigma F7425 0.8 mg/ml 1/5000 0.16
  FlagM21 Monoclonal Sigma F3165 5 mg/ml 1/12500 0.4
V5 Anti-V53 Monoclonal Invitrogen R960-25 1.07 mg/ml 1/12500 0.0856
Myc Anti-Myc2
Polyclonal
Polyclonal Upstate 06-549 1 mg/ml 1/5000 0.2
  9E103 Monoclonal Santa Cruz Sc-40 0.2 mg/ml 1/1000 0.2
HA HA 113 Monoclonal Covance MMS-101R 2-3 mg/ml 1/25000 0.1
  12CA53 Monoclonal Roch Applied 11 583 816 001 0.4 mg/ml 1/5000 0.08
AU1 Anti-AU13 Monoclonal Covance MMS-130R 5-7 mg/ml 1/5000 1.2
GFP Anti-eGFP4
Polyclonal
Polyclonal In-house - - 1/10000 -
  1. 1 affinity purified IgGs using a column bearing the inmmunizing peptide
  2. 2 affinity purified IgGs using a column bearing protein A
  3. 3 purification method not specified
  4. 4 not purified
  5. In order to compare antibody dilutions, the optimal dilutions (based on current study) for IHC reckoned with the antibody concentration are mentioned in this table.