Skip to main content

Table 2 Detailed overview of characterized antibodies

From: Immunohistochemical detection of transgene expression in the brain using small epitope tags

Epitope

Antibody

Clonality

Company

Product N°

Concentration

Optimal dilution for IHC based on current study

Optimal concentration (μg/ml) for IHC based on current study

3flag and flag

Anti-flag polyclonal1

Polyclonal

Sigma

F7425

0.8 mg/ml

1/5000

0.16

 

FlagM21

Monoclonal

Sigma

F3165

5 mg/ml

1/12500

0.4

V5

Anti-V53

Monoclonal

Invitrogen

R960-25

1.07 mg/ml

1/12500

0.0856

Myc

Anti-Myc2

Polyclonal

Polyclonal

Upstate

06-549

1 mg/ml

1/5000

0.2

 

9E103

Monoclonal

Santa Cruz

Sc-40

0.2 mg/ml

1/1000

0.2

HA

HA 113

Monoclonal

Covance

MMS-101R

2-3 mg/ml

1/25000

0.1

 

12CA53

Monoclonal

Roch Applied

11 583 816 001

0.4 mg/ml

1/5000

0.08

AU1

Anti-AU13

Monoclonal

Covance

MMS-130R

5-7 mg/ml

1/5000

1.2

GFP

Anti-eGFP4

Polyclonal

Polyclonal

In-house

-

-

1/10000

-

  1. 1 affinity purified IgGs using a column bearing the inmmunizing peptide
  2. 2 affinity purified IgGs using a column bearing protein A
  3. 3 purification method not specified
  4. 4 not purified
  5. In order to compare antibody dilutions, the optimal dilutions (based on current study) for IHC reckoned with the antibody concentration are mentioned in this table.