Figure 6

Integration of GRQCIMFIGFP. Genomic DNA was extracted from NIH3T3 cells transduced with GRQCIMFIGFP virus. PCR on genomic DNA shows that retroviral genes were integrated into the genome of cells. (A) The retroviral construct and the places where the different primers bind within the retrovirus construct. (B) An electrophoresis gel of the PCR product from genomic DNA (lanes 2 and 5) and plasmid DNA (lanes 3 and 4). Using primers 1f and 1r and then 1f' and 1r' to clone out a 1029 bp band (lanes 2 and 3). Primers 2f and 2r and then 2f' and 2r' were used to clone out a 1650 bp band (lanes 4 and 5). Lane is an l kb molecular weight marker. Figure (C and D) are pictures of NIH3T3 cells transduced with GRQCIMFIGFP with (C) no ligand and (D) 10 μM LG335.