Figure 4

Ligand Time Course. HEK293T cells were transfected with pMSCVGRQCIMFI and p17*4TataLuc and 8 hours after transfection 100 nM of LG335 was added to cells. Cells were harvested every 8 hours for the ligand time course. Grey bars represent the addition of LG335 8 hours after transfection. The black bars represent the addition of LG335 8 hours after transfection and the removal of ligand 32 hours after. The white bars represent cells transfected with plasmid, but no ligand was added.