Figure 4

SDS-PAGE analysis of SUMO-FGF21 by SUMO protease and rFGF21 assay by Western blot. The purified SUMO-FGF21 was digested by SUMO protease at 4°C overnight. As seen in Figure 4A, rFGF21 was cut from the SUMO-FGF21 by SUMO protease. Lane1: Protein Molecular Weight Marker. Lane2: purified rFGF21;Lane3: SUMO-FGF21 fusion protein after treatment with SUMO protease. Western blot analysis was performed as described in Materials and Methods. Immunoacitivity of purified rFGF21 with anti-human FGF21 antibody is shown in Figure 4B. Lane1: standard FGF21 (PeproTech Comp.);Lane2: purified rFGF21.