Figure 2

Visualization of Cy3-labeled dsRNA electroporated into unfed I. scapularis nymphs: A) ventral view, unfed nymph electroporated with Cy3 dye (10×); B) dorsal view of nymphal tick electroporated with Cy3 dye after 24 hrs feeding on mouse (5×); C) dorsal view of nymphal tick electroporated with Cy3 dye after 36 hrs feeding on mouse (10×); D) Ventral view of nymphal tick electroporated with Cy3 labeled GAPDH control siRNA after 48 hrs feeding on mouse (10×); E) Ventral view of 48 hrs fed nymph electroporated with Cy3 labeled Actin dsRNA (10×); F) dorsal view of 48 hrs fed nymph electroporated with Cy3 labeled β-Actin dsRNA; and G) Cy3 labeled Actin dsRNA in the dissected tick salivary gland acini (20×). Arrows indicate the detection of electroporated dsRNA all over the nymphal ticks.