TY - JOUR AU - Arakawa, Hiroshi AU - Lodygin, Dimitry AU - Buerstedde, Jean-Marie PY - 2001 DA - 2001/09/26 TI - Mutant loxP vectors for selectable marker recycle and conditional knock-outs JO - BMC Biotechnology SP - 7 VL - 1 IS - 1 AB - Gene disruption by targeted integration of transfected constructs becomes increasingly popular for studies of gene function. The chicken B cell line DT40 has been widely used as a model for gene knock-outs due to its high targeted integration activity. Disruption of multiple genes and complementation of the phenotypes is, however, restricted by the number of available selectable marker genes. It is therefore highly desirable to recycle the selectable markers using a site-specific recombination system like Cre/loxP. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-1-7 DO - 10.1186/1472-6750-1-7 ID - Arakawa2001 ER -