RNA | # Picked | # Binders | # Specific | Signal | Signal |
---|
Target | | (%) | Binder (%) | Ratios/SA | Ratios/RNA |
---|
APP | 240 | 186 (78) | 21 (11) | 19.6 | 7.4 |
HCV | 240 | 207 (86) | 31 (15) | 16.7 | 7.9 |
IGF-1 | 240 | 184 (77) | 9 (5) | 18.7 | 3.1 |
RRE | 48 | 25 (42) | 4 (16) | 25.3 | 2.3 |
- Individual clones were picked after three to four rounds of panning of random peptide displayed phage libraries for four different RNA targets. Phage rescued from these clones were tested for binding to the specific RNA target and an irrelevant RNA target. Clones that had a binding ratio of ≥ 2.0 over streptavidin (SA) background were categorized as binders. Binders were then sub-categorized as specific binders if they had a binding ratio of target:irrelevant ≥ 2.0.