Skip to main content

Advertisement

Table 3 Results of panning experiments on selected mRNA targets using the primary phage displayed peptide libraries

From: A surrogate-based approach for post-genomic partner identification

RNA # Picked # Binders # Specific Signal Signal
Target   (%) Binder (%) Ratios/SA Ratios/RNA
APP 240 186 (78) 21 (11) 19.6 7.4
HCV 240 207 (86) 31 (15) 16.7 7.9
IGF-1 240 184 (77) 9 (5) 18.7 3.1
RRE 48 25 (42) 4 (16) 25.3 2.3
  1. Individual clones were picked after three to four rounds of panning of random peptide displayed phage libraries for four different RNA targets. Phage rescued from these clones were tested for binding to the specific RNA target and an irrelevant RNA target. Clones that had a binding ratio of ≥ 2.0 over streptavidin (SA) background were categorized as binders. Binders were then sub-categorized as specific binders if they had a binding ratio of target:irrelevant ≥ 2.0.