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Table 1 The effects of salt concentration and the pH of phosphate buffer on the protein binding to MEL-A attached onto polyHEMA beads

From: Mannosylerythritol lipid, a yeast extracellular glycolipid, shows high binding affinity towards human immunoglobulin G

   Amount of protein bound
   (mg/g composite a)
Salt(M) pH HIgG HSA
Control (0) 7.0 2.06 0.76
Na2SO4 (1.0) 4.0 3.93 1.95
  5.6 3.63 1.12
  6.4 3.45 0.73
  7.0 2.87 1.20
  7.5 3.73 1.28
NaCl (1.0) 7.0 2.35 0.53
  1. a Composite bearing 2.99 mg of MEL-A per g of polyHEMA. The protein (1.0 mg) was added to the mixture of MEL-polyHEMA composite and 50 mM phosphate buffer (3 ml) in a polypropylene tube. The tube was incubated for 1 hr and then centrifuged. The amount of bound protein was calculated by subtracting that of unbound protein from the total added; the amount of unbound protein was estimated by measuring the UV absorbance of the supernatant. Each data is the mean of triplicate. MEL-A, mannosylerythritol lipid-A; polyHEMA, poly (2-hydroxyethyl methacylate); HIgG, human immunoglobulin G; HSA, human serum albumin.