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Table 1 Summary of selection for "Specialist" and "Generalist" polymerases

From: A combined in vitro / in vivo selection for polymerases with novel promoter specificities

A. Selection of "Specialist" (T3 like polymerase)
  §Number of variants Polymerase sequence* Promoter sequence (-11 to -8)
   746 747 748  
Round – 1 4 R (CGA) L (TTG) D (GAT) CACT
  1 R (CGT) L (TTA) Q (CAG) CACT
  1 R (CGG) L (TTA) S (TCT) CACT
  1 R (CGG) C (TGT) S (TCT) CACT
  1 R (CGC) P (CCC) E(GAA) CACT
  1 R (AGG) T (ACA) N(AAC) CACT
Round – 2 17 R (CGA) L (TTA) D (GAT) CACT
  3 R (CGA) L (TTG) Q (CAG) CACT
Round – 3 16 R (CGA) L (TTA) D (GAT) CACT
* wild type sequence: R (CGC) L (TTG) N (AAC) GACT
B. Selection of the "Generalist" polymerase
  §Number of variants Polymerase sequence* Promoter sequence (-11 to -8)
   748 756 758  
Round – 3      
R3–9 3 N(AAT) R (CGT) C (TGC) GACT(wt)
R3–32 3 N(AAT) R (CGT) C (TGC) GACG
R3–26 1 N(AAT) R (CGT) C (TGC) TGTA
R3–17 1 A (GCC) M (ATG) S (AGT) GGTA
R3–5 1 F (TTC) G (GGC) I (ATA) G CTA
R3–29 1 T (ACG) K(AAG) Q (CAA) GACT
Round – 4      
R4–14 7 N(AAT) R (CGT) C (TGC) GACT
R4–16 3 N(AAT) R (CGT) C (TGC) GTTA
R4–17 2 N(AAT) R (CGT) C (TGC) GTCA
ppC6 2 N(AAT) R (CGT) C (TGC) GATA
R4–11 3 N(AAT) R (CGA) S (AGT) GACT
* wild type sequence N (AAC) R (CGC) Q (CAG) GACT
  1. §number of times the particular variant was isolated.