Figure 2From: Generation of single-copy transgenic mouse embryos directly from ES cells by tetraploid embryo complementationEmbryos derived from Hprt-deficient ES cells by tetraploid aggregation. A-C) Photographs of embryos at E13.0 derived from F3 cells. Pigmentation can be seen in the eyes (arrow) reflecting their ES cell origin. D) Southern blot analyses of BamH1-digested genomic DNA isolated from five (#1 – #5) F3 ES cell-derived embryos that was hybridized to a radiolabeled intron 3 probe. Only the mutant allele is detected in F3 ES cell derived embryos. The wild type allele can be seen in female F3 offspring as discussed in the text.Back to article page