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Table 1 Summary of GS-E induction of luciferase activity in clone MBT-2 + RHeoSwitch #2 + pG5-luc – 5B3

From: Manipulation of gene expression by an ecdysone-inducible gene switch in tumor xenografts

Experiment

RLU 1^

RLU 1

 

RLU 2'

RLU 2

 

RLU1/RLU2

RLU 1/RLU2

 
 

CON

GS-E

T/C

CON

GS-E

T/C

CON

GS-E

T/C

A

4457*

992343

222.6

543227

678517

1.2

0.01

1.46

178.3

B

4123

948272

230.0

579818

709519

1.2

0.01

1.34

188.0

C

4771

982058

205.8

658164

823060

1.3

0.01

1.19

164.6

D

3915

597044

152.5

537789

720480

1.3

0.01

0.83

113.8

E

1757

350947

199.7

75614

95031

1.3

0.02

3.69

158.9

F

1405

314349

223.7

232147

300061

1.3

0.01

1.05

173.1

G

1343

234858

174.9

174883

207357

1.2

0.01

1.13

147.5

H

3958

737902

186.4

197599

227480

1.2

0.02

3.24

161.9

I

3822

710260

185.8

209591

251976

1.2

0.02

2.82

154.6

J

3714

692231

186.4

218191

259666

1.2

0.02

2.67

156.6

  

Mean

196.8

 

Mean

1.2

 

Mean

159.7

  

SD

24.4

 

SD

0.1

 

SD

20.1

  

N

10

 

N

10

 

N

10

  1. CON: control carrier applied only Only 48 hour time-points displayed in this summary ^RLU1 is expression of firefly luciferase from the inducible promoter 'RLU2 is expression of Renilla luciferase from the constitutive TK promoter *All data points shown are the average from duplicate or triplicate samples