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  1. Research Article

    Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation

    Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are su...

    Tingting Shang, Dayong Si, Dongyan Zhang, Xuhui Liu, Longmei Zhao, Cong Hu, Yu Fu and Rijun Zhang

    BMC Biotechnology 2017 17:55

    Published on: 21 June 2017

  2. Methodology article

    Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

    Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated...

    Mayu Yasunaga, Yasuko Fujita, Rumiko Saito, Mitsuo Oshimura and Yoshihiro Nakajima

    BMC Biotechnology 2017 17:54

    Published on: 20 June 2017

  3. Research article

    Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression

    The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simu...

    Michael Puckette, Thomas Burrage, John G. Neilan and Max Rasmussen

    BMC Biotechnology 2017 17:52

    Published on: 12 June 2017

  4. Research article

    Somatic cell selection for chlorsulfuron-resistant mutants in potato: identification of point mutations in the acetohydroxyacid synthase gene

    Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in ...

    Philippa J. Barrell, Julie M. Latimer, Samantha J. Baldwin, Michelle L. Thompson, Jeanne M.E. Jacobs and Anthony J. Conner

    BMC Biotechnology 2017 17:49

    Published on: 6 June 2017

  5. Research article

    Fermentation of lactose to ethanol in cheese whey permeate and concentrated permeate by engineered Escherichia coli

    Whey permeate is a lactose-rich effluent remaining after protein extraction from milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation can complete whey valorization chain by...

    Lorenzo Pasotti, Susanna Zucca, Michela Casanova, Giuseppina Micoli, Maria Gabriella Cusella De Angelis and Paolo Magni

    BMC Biotechnology 2017 17:48

    Published on: 2 June 2017

  6. Research article

    Biochemical characterization of the xylan hydrolysis profile of the extracellular endo-xylanase from Geobacillus thermodenitrificans T12

    Endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. Hemicellulose degradation by Geobacillus spp. is facilitated by the hemicellulose utilization (HUS) locus that is pre...

    Martinus J.A. Daas, Patricia Murciano Martínez, Antonius H.P. van de Weijer, John van der Oost, Willem M. de Vos, Mirjam A. Kabel and Richard van Kranenburg

    BMC Biotechnology 2017 17:44

    Published on: 18 May 2017

  7. Methodology article

    AAV vector distribution in the mouse respiratory tract following four different methods of administration

    Targeted delivery of gene therapy vectors to the mouse respiratory tract is often performed via intranasal or intratracheal administration; however, there can be a great deal of variability between these metho...

    Lisa A. Santry, Joelle C. Ingrao, Darrick L. Yu, Jondavid G. de Jong, Laura P. van Lieshout, Geoffrey A. Wood and Sarah K. Wootton

    BMC Biotechnology 2017 17:43

    Published on: 15 May 2017

  8. Research article

    Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells

    Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastas...

    Stefan Somplatzki, Martina Mühlenhoff, Andrea Kröger, Rita Gerardy-Schahn and Thomas Böldicke

    BMC Biotechnology 2017 17:42

    Published on: 12 May 2017

  9. Research article

    Pollen-mediated gene flow from transgenic to non-transgenic switchgrass (Panicum virgatum L.) in the field

    Switchgrass is C4 perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter...

    Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea R. Johnson, Micaha Hanson, Jason N. Burris, Charles Kwit and C. Neal Stewart

    BMC Biotechnology 2017 17:40

    Published on: 2 May 2017

  10. Research article

    Distinctive molecular and biochemical characteristics of a glycoside hydrolase family 20 β-N-acetylglucosaminidase and salt tolerance

    Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acety...

    Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han and Zunxi Huang

    BMC Biotechnology 2017 17:37

    Published on: 11 April 2017

  11. Research article

    Improved n-butanol production via co-expression of membrane-targeted tilapia metallothionein and the clostridial metabolic pathway in Escherichia coli

    N-Butanol has favorable characteristics for use as either an alternative fuel or platform chemical. Bio-based n-butanol production using microbes is an emerging technology that requires further development. Al...

    Wei-Chih Chin, Kuo-Hsing Lin, Chun-Chi Liu, Kenji Tsuge and Chieh-Chen Huang

    BMC Biotechnology 2017 17:36

    Published on: 11 April 2017

  12. Research article

    Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

    Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which t...

    Nadezhda A. Orlova, Sergey V. Kovnir, Alexandre G. Gabibov and Ivan I. Vorobiev

    BMC Biotechnology 2017 17:33

    Published on: 24 March 2017

  13. Methodology article

    A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends

    Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction s...

    Fanli Zeng, Zhimin Hao, Pan Li, Yanan Meng, Jingao Dong and Yibin Lin

    BMC Biotechnology 2017 17:32

    Published on: 17 March 2017

  14. Research article

    Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship

    Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k ...

    Qing Wang, Luyao Bao, Chenjun Jia, Mei Li, Jian-Jun Li and Xuefeng Lu

    BMC Biotechnology 2017 17:31

    Published on: 16 March 2017

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