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  1. Research article

    A fast and effective determination of the biodistribution and subcellular localization of fluorescent immunoliposomes in freshly excised animal organs

    Preclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated...

    Felista L. Tansi, Ronny Rüger, Ansgar M. Kollmeier, Claudia Böhm, Roland E. Kontermann, Ulf K. Teichgraeber, Alfred Fahr and Ingrid Hilger

    BMC Biotechnology 2017 17:8

    Published on: 18 January 2017

  2. Research article

    Improved genome sequencing using an engineered transposase

    Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transp...

    Amirali Kia, Christian Gloeckner, Trina Osothprarop, Niall Gormley, Erin Bomati, Michelle Stephenson, Igor Goryshin and Molly Min He

    BMC Biotechnology 2017 17:6

    Published on: 17 January 2017

  3. Research article

    The antibacterial activity and mechanism of ginkgolic acid C15:1

    The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains.

    Zhebin Hua, Caie Wu, Gongjian Fan, Zhenxing Tang and Fuliang Cao

    BMC Biotechnology 2017 17:5

    Published on: 14 January 2017

  4. Research article

    Production of human pro-relaxin H2 in the yeast Pichia pastoris

    Initially known as the reproductive hormone, relaxin was shown to possess other therapeutically useful properties that include extracellular matrix remodeling, anti-inflammatory, anti-ischemic and angiogenic e...

    D. Cimini, K. Della Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. V. A. Pirozzi, F. Ferrara, R. Formisano, M. De Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone and C. Schiraldi

    BMC Biotechnology 2017 17:4

    Published on: 14 January 2017

  5. Methodology article

    Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

    There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied ...

    Laure-Hélène Ouisse, Laetitia Gautreau-Rolland, Marie-Claire Devilder, Michael Osborn, Melinda Moyon, Jonathan Visentin, Frank Halary, Marianne Bruggemann, Roland Buelow, Ignacio Anegon and Xavier Saulquin

    BMC Biotechnology 2017 17:3

    Published on: 9 January 2017

  6. Research article

    Multi-antigen avian influenza a (H7N9) virus-like particles: particulate characterizations and immunogenicity evaluation in murine and avian models

    Human infection with avian influenza A virus (H7N9) was first reported in China in March 2013. Since then, hundreds of cases have been confirmed showing severe symptoms with a high mortality rate. The virus wa...

    Che-Ming Jack Hu, Chu-Yang Chien, Ming-Tsan Liu, Zih-Syun Fang, Sui-Yuan Chang, Rong-Huay Juang, Shih-Chung Chang and Hui-Wen Chen

    BMC Biotechnology 2017 17:2

    Published on: 7 January 2017

  7. Methodology article

    New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag

    Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affi...

    Marco Biancucci, Jazel S. Dolores, Jennifer Wong, Sarah Grimshaw, Wayne F. Anderson, Karla J. F. Satchell and Keehwan Kwon

    BMC Biotechnology 2017 17:1

    Published on: 5 January 2017

  8. Research article

    Means of enhancing bone fracture healing: optimal cell source, isolation methods and acoustic stimulation

    The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long bone fractures of the lower limbs cannot be restored without intervention and often lead to n...

    Corina Adriana Ghebes, Maaike Vera Jasmijn Braham, Adelgunde Veronica Clemens Maria Zeegers, Auke Jan Sijbe Renard, Hugo Fernandes and Daniel B F Saris

    BMC Biotechnology 2016 16:89

    Published on: 12 December 2016

  9. Methodology article

    Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

    Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenes...

    Ana Filipa Sequeira, Joana L. A. Brás, Catarina I. P. D. Guerreiro, Renaud Vincentelli and Carlos M. G. A. Fontes

    BMC Biotechnology 2016 16:86

    Published on: 1 December 2016

  10. Research article

    Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application

    The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have de...

    Luciano Lucas Guerra, Natalia Inés Faccinetti, Aldana Trabucchi, Bruno David Rovitto, Adriana Victoria Sabljic, Edgardo Poskus, Ruben Francisco Iacono and Silvina Noemí Valdez

    BMC Biotechnology 2016 16:84

    Published on: 24 November 2016

  11. Methodology article

    Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization

    Due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (DENV), chikungunya and zika virus, have developed into major public health challenges....

    Natalie Rose, Carlos Augusto Pinho-Nascimento, Alessia Ruggieri, Paola Favuzza, Marco Tamborrini, Hanna Roth, Marcia Terezinha Baroni de Moraes, Hugues Matile, Thomas Jänisch, Gerd Pluschke and Katharina Röltgen

    BMC Biotechnology 2016 16:83

    Published on: 22 November 2016

  12. Research article

    Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification

    Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their compo...

    Wanwitoo Wanmolee, Warasirin Sornlake, Nakul Rattanaphan, Surisa Suwannarangsee, Navadol Laosiripojana and Verawat Champreda

    BMC Biotechnology 2016 16:82

    Published on: 21 November 2016

  13. Research article

    Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk

    Tobacco stalk is one kind of abundant crop residues in China. The high lignification of tobacco stalk increases its reusing cost and the existing of nicotine will cause serious pollution. The biodegradation of...

    Yulong Su, He Xian, Sujuan Shi, Chengsheng Zhang, S. M. Nuruzzaman Manik, Jingjing Mao, Ge Zhang, Weihong Liao, Qian Wang and Haobao Liu

    BMC Biotechnology 2016 16:81

    Published on: 21 November 2016

  14. Research article

    Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases

    Hexuronic acids such as D-galacturonic acid and D-glucuronic acid can be utilized via different pathways within the metabolism of microorganisms. One representative, the oxidative pathway, generates α-keto-glu...

    André Pick, Barbara Beer, Risa Hemmi, Rena Momma, Jochen Schmid, Kenji Miyamoto and Volker Sieber

    BMC Biotechnology 2016 16:80

    Published on: 17 November 2016

  15. Research article

    A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum

    Pyruvate kinase (Pyk) catalyzes the generation of pyruvate and ATP in glycolysis and functions as a key switch in the regulation of carbon flux distribution. Both the substrates and products of Pyk are involve...

    Xin Chai, Xiuling Shang, Yu Zhang, Shuwen Liu, Yong Liang, Yun Zhang and Tingyi Wen

    BMC Biotechnology 2016 16:79

    Published on: 16 November 2016

  16. Research article

    Repair of cartilage defects in osteoarthritis rats with induced pluripotent stem cell derived chondrocytes

    The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA)...

    Yanxia Zhu, Xiaomin Wu, Yuhong Liang, Hongsheng Gu, Kedong Song, Xuenong Zou and Guangqian Zhou

    BMC Biotechnology 2016 16:78

    Published on: 9 November 2016

  17. Research article

    Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis

    Family 11 alkaline xylanases have great potential economic applications in the pulp and paper industry. In this study, we would improve the alkalophilicity of family 11 alkaline xylanase Xyn11A-LC from Bacillus s...

    Wenqin Bai, Yufan Cao, Jun Liu, Qinhong Wang and Zhenhu Jia

    BMC Biotechnology 2016 16:77

    Published on: 8 November 2016

  18. Methodology article

    Sequential and counter-selectable cassettes for fission yeast

    Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of i...

    Hanna Amelina, Vera Moiseeva, Laura Catharine Collopy, Siân Rosanna Pearson, Christine Anne Armstrong and Kazunori Tomita

    BMC Biotechnology 2016 16:76

    Published on: 8 November 2016

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