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  1. Methodology article

    Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)

    Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have...

    Jun-Zheng Wu, Qin Liu, Xiao-Shan Geng, Kai-Mian Li, Li-Juan Luo and Jin-Ping Liu

    BMC Biotechnology 2017 17:29

    Published on: 14 March 2017

  2. Research article

    The role of community engagement in the adoption of new agricultural biotechnologies by farmers: the case of the Africa harvest tissue-culture banana in Kenya

    The tissue culture banana (TCB) is a biotechnological agricultural innovation that has been adopted widely in commercial banana production. In 2003, Africa Harvest Biotech Foundation International (AH) initiat...

    Sunita V. S. Bandewar, Florence Wambugu, Emma Richardson and James V. Lavery

    BMC Biotechnology 2017 17:28

    Published on: 13 March 2017

  3. Research article

    An engineered food-grade Lactococcus lactis strain for production and delivery of heat-labile enterotoxin B subunit to mucosal sites

    Recent researches have been focusing on mucosal immune adjuvants, which play the key roles in mucosal immunization and have become the limitation for non-injected vaccine development. Escherichia coli heat-labile...

    Nan Sun, Rongguang Zhang, Guangcai Duan, Xiaoyan Peng, Chen Wang, Qingtang Fan, Shuaiyin Chen and Yuanlin Xi

    BMC Biotechnology 2017 17:25

    Published on: 6 March 2017

  4. Research article

    Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus for baking industry and its magnetic immobilization

    α-Amylase plays a pivotal role in a broad range of industrial processes. To meet increasing demands of biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. Howe...

    Lei He, Youzhi Mao, Lujia Zhang, Hualei Wang, Siti Aisyah Alias, Bei Gao and Dongzhi Wei

    BMC Biotechnology 2017 17:22

    Published on: 28 February 2017

  5. Research article

    Constitutive expression of active microbial transglutaminase in Escherichia coli and comparative characterization to a known variant

    Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and b...

    Gabe Javitt, Zohar Ben-Barak-Zelas, Moran Jerabek-Willemsen and Ayelet Fishman

    BMC Biotechnology 2017 17:23

    Published on: 28 February 2017

  6. Research article

    Enhancing the thermostability of α-L-rhamnosidase from Aspergillus terreus and the enzymatic conversion of rutin to isoquercitrin by adding sorbitol

    Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability...

    Lin Ge, Anna Chen, Jianjun Pei, Linguo Zhao, Xianying Fang, Gang Ding, Zhenzhong Wang, Wei Xiao and Feng Tang

    BMC Biotechnology 2017 17:21

    Published on: 27 February 2017

  7. Research article

    Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol

    Manganese peroxidase (MnP) of white rot basidiomycetes, an extracellular heme enzyme, is part of a peroxidase superfamily that is capable of degrading the different phenolic compounds. Ganoderma, a white rot basi...

    Hui Xu, Meng-Yuan Guo, Yan-Hua Gao, Xiao-Hui Bai and Xuan-Wei Zhou

    BMC Biotechnology 2017 17:19

    Published on: 23 February 2017

  8. Research article

    Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

    poly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-sca...

    Jun Xia, Jiaxing Xu, Xiaoyan Liu, Jiming Xu, Xingfeng Wang and Xiangqian Li

    BMC Biotechnology 2017 17:20

    Published on: 23 February 2017

  9. Research article

    Evaluating the expression profile and stability of different UCOE containing vector combinations in mAb-producing CHO cells

    As the demand for monoclonal antibodies (mAb) increases, more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cel...

    Fatemeh Nematpour, Fereidoun Mahboudi, Behrouz Vaziri, Vahid Khalaj, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat and Fatemeh Davami

    BMC Biotechnology 2017 17:18

    Published on: 22 February 2017

  10. Research article

    Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system

    Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs. As chicken primordial germ cells (PGCs) have been reported a...

    Xiaochuan Tang, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu and Honglin Liu

    BMC Biotechnology 2017 17:17

    Published on: 20 February 2017

  11. Research article

    Penicillin production in industrial strain Penicillium chrysogenum P2niaD18 is not dependent on the copy number of biosynthesis genes

    Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many pr...

    Sandra Ziemons, Katerina Koutsantas, Kordula Becker, Tim Dahlmann and Ulrich Kück

    BMC Biotechnology 2017 17:16

    Published on: 16 February 2017

  12. Research article

    Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring

    Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-pol...

    Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua and Duriya Chantasingh

    BMC Biotechnology 2017 17:15

    Published on: 16 February 2017

  13. Research article

    Mild electrical stimulation with heat shock guides differentiation of embryonic stem cells into Pdx1-expressing cells within the definitive endoderm

    Because of the increasing number of diabetic patients, it is important to generate pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing cells, which are capable of differentiating into pancreatic endocrin...

    Tomoaki Koga, Nobuaki Shiraki, Shuichiro Yano, Mary Ann Suico, Saori Morino-Koga, Takashi Sato, Tsuyoshi Shuto, Shoen Kume and Hirofumi Kai

    BMC Biotechnology 2017 17:14

    Published on: 15 February 2017

  14. Methodology article

    Automated freeze-thaw cycles for decellularization of tendon tissue - a pilot study

    Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of e...

    Susanne Pauline Roth, Sina Marie Glauche, Amelie Plenge, Ina Erbe, Sandra Heller and Janina Burk

    BMC Biotechnology 2017 17:13

    Published on: 14 February 2017

  15. Research article

    Inducing goat pluripotent stem cells with four transcription factor mRNAs that activate endogenous promoters

    Traditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exoge...

    Hao Chen, Qisheng Zuo, Yingjie Wang, Jiuzhou Song, Huilin Yang, Yani Zhang and Bichun Li

    BMC Biotechnology 2017 17:11

    Published on: 13 February 2017

  16. Research article

    Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments

    Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As...

    Aiswarya Chenthamarakshan, Nayana Parambayil, Nafeesathul Miziriya, P. S. Soumya, M. S. Kiran Lakshmi, Anala Ramgopal, Anuja Dileep and Padma Nambisan

    BMC Biotechnology 2017 17:12

    Published on: 13 February 2017

  17. Research article

    Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectors

    RNAi (RNA interference) is a technology for silencing of target genes via sequence-specific manner. RNAi technology has been used for development of anti-pathogenic crops. In 2007, development of transgenic plant...

    Oyunchuluun Ganbaatar, Budao Cao, Yanan Zhang, Duran Bao, Wenhua Bao and Hada Wuriyanghan

    BMC Biotechnology 2017 17:9

    Published on: 9 February 2017

  18. Research article

    Transcriptomic response of wolf spider, Pardosa pseudoannulata, to transgenic rice expressing Bacillus thuringiensis Cry1Ab protein

    Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthet...

    Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song and Xuguo Zhou

    BMC Biotechnology 2017 17:7

    Published on: 18 January 2017

  19. Research article

    A fast and effective determination of the biodistribution and subcellular localization of fluorescent immunoliposomes in freshly excised animal organs

    Preclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated...

    Felista L. Tansi, Ronny Rüger, Ansgar M. Kollmeier, Claudia Böhm, Roland E. Kontermann, Ulf K. Teichgraeber, Alfred Fahr and Ingrid Hilger

    BMC Biotechnology 2017 17:8

    Published on: 18 January 2017

  20. Research article

    Improved genome sequencing using an engineered transposase

    Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transp...

    Amirali Kia, Christian Gloeckner, Trina Osothprarop, Niall Gormley, Erin Bomati, Michelle Stephenson, Igor Goryshin and Molly Min He

    BMC Biotechnology 2017 17:6

    Published on: 17 January 2017

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