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  1. Research article

    Mild electrical stimulation with heat shock guides differentiation of embryonic stem cells into Pdx1-expressing cells within the definitive endoderm

    Because of the increasing number of diabetic patients, it is important to generate pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing cells, which are capable of differentiating into pancreatic endocrin...

    Tomoaki Koga, Nobuaki Shiraki, Shuichiro Yano, Mary Ann Suico, Saori Morino-Koga, Takashi Sato, Tsuyoshi Shuto, Shoen Kume and Hirofumi Kai

    BMC Biotechnology 2017 17:14

    Published on: 15 February 2017

  2. Methodology article

    Automated freeze-thaw cycles for decellularization of tendon tissue - a pilot study

    Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of e...

    Susanne Pauline Roth, Sina Marie Glauche, Amelie Plenge, Ina Erbe, Sandra Heller and Janina Burk

    BMC Biotechnology 2017 17:13

    Published on: 14 February 2017

  3. Research article

    Inducing goat pluripotent stem cells with four transcription factor mRNAs that activate endogenous promoters

    Traditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exoge...

    Hao Chen, Qisheng Zuo, Yingjie Wang, Jiuzhou Song, Huilin Yang, Yani Zhang and Bichun Li

    BMC Biotechnology 2017 17:11

    Published on: 13 February 2017

  4. Research article

    Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments

    Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As...

    Aiswarya Chenthamarakshan, Nayana Parambayil, Nafeesathul Miziriya, P. S. Soumya, M. S. Kiran Lakshmi, Anala Ramgopal, Anuja Dileep and Padma Nambisan

    BMC Biotechnology 2017 17:12

    Published on: 13 February 2017

  5. Research article

    Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectors

    RNAi (RNA interference) is a technology for silencing of target genes via sequence-specific manner. RNAi technology has been used for development of anti-pathogenic crops. In 2007, development of transgenic plant...

    Oyunchuluun Ganbaatar, Budao Cao, Yanan Zhang, Duran Bao, Wenhua Bao and Hada Wuriyanghan

    BMC Biotechnology 2017 17:9

    Published on: 9 February 2017

  6. Research article

    Transcriptomic response of wolf spider, Pardosa pseudoannulata, to transgenic rice expressing Bacillus thuringiensis Cry1Ab protein

    Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthet...

    Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song and Xuguo Zhou

    BMC Biotechnology 2017 17:7

    Published on: 18 January 2017

  7. Research article

    A fast and effective determination of the biodistribution and subcellular localization of fluorescent immunoliposomes in freshly excised animal organs

    Preclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated...

    Felista L. Tansi, Ronny Rüger, Ansgar M. Kollmeier, Claudia Böhm, Roland E. Kontermann, Ulf K. Teichgraeber, Alfred Fahr and Ingrid Hilger

    BMC Biotechnology 2017 17:8

    Published on: 18 January 2017

  8. Research article

    Improved genome sequencing using an engineered transposase

    Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transp...

    Amirali Kia, Christian Gloeckner, Trina Osothprarop, Niall Gormley, Erin Bomati, Michelle Stephenson, Igor Goryshin and Molly Min He

    BMC Biotechnology 2017 17:6

    Published on: 17 January 2017

  9. Research article

    The antibacterial activity and mechanism of ginkgolic acid C15:1

    The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains.

    Zhebin Hua, Caie Wu, Gongjian Fan, Zhenxing Tang and Fuliang Cao

    BMC Biotechnology 2017 17:5

    Published on: 14 January 2017

  10. Research article

    Production of human pro-relaxin H2 in the yeast Pichia pastoris

    Initially known as the reproductive hormone, relaxin was shown to possess other therapeutically useful properties that include extracellular matrix remodeling, anti-inflammatory, anti-ischemic and angiogenic e...

    D. Cimini, K. Della Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. V. A. Pirozzi, F. Ferrara, R. Formisano, M. De Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone and C. Schiraldi

    BMC Biotechnology 2017 17:4

    Published on: 14 January 2017

  11. Methodology article

    Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

    There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied ...

    Laure-Hélène Ouisse, Laetitia Gautreau-Rolland, Marie-Claire Devilder, Michael Osborn, Melinda Moyon, Jonathan Visentin, Frank Halary, Marianne Bruggemann, Roland Buelow, Ignacio Anegon and Xavier Saulquin

    BMC Biotechnology 2017 17:3

    Published on: 9 January 2017

  12. Research article

    Multi-antigen avian influenza a (H7N9) virus-like particles: particulate characterizations and immunogenicity evaluation in murine and avian models

    Human infection with avian influenza A virus (H7N9) was first reported in China in March 2013. Since then, hundreds of cases have been confirmed showing severe symptoms with a high mortality rate. The virus wa...

    Che-Ming Jack Hu, Chu-Yang Chien, Ming-Tsan Liu, Zih-Syun Fang, Sui-Yuan Chang, Rong-Huay Juang, Shih-Chung Chang and Hui-Wen Chen

    BMC Biotechnology 2017 17:2

    Published on: 7 January 2017

  13. Methodology article

    New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag

    Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affi...

    Marco Biancucci, Jazel S. Dolores, Jennifer Wong, Sarah Grimshaw, Wayne F. Anderson, Karla J. F. Satchell and Keehwan Kwon

    BMC Biotechnology 2017 17:1

    Published on: 5 January 2017

  14. Research article

    Means of enhancing bone fracture healing: optimal cell source, isolation methods and acoustic stimulation

    The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long bone fractures of the lower limbs cannot be restored without intervention and often lead to n...

    Corina Adriana Ghebes, Maaike Vera Jasmijn Braham, Adelgunde Veronica Clemens Maria Zeegers, Auke Jan Sijbe Renard, Hugo Fernandes and Daniel B F Saris

    BMC Biotechnology 2016 16:89

    Published on: 12 December 2016

  15. Methodology article

    Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

    Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenes...

    Ana Filipa Sequeira, Joana L. A. Brás, Catarina I. P. D. Guerreiro, Renaud Vincentelli and Carlos M. G. A. Fontes

    BMC Biotechnology 2016 16:86

    Published on: 1 December 2016

  16. Research article

    Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application

    The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have de...

    Luciano Lucas Guerra, Natalia Inés Faccinetti, Aldana Trabucchi, Bruno David Rovitto, Adriana Victoria Sabljic, Edgardo Poskus, Ruben Francisco Iacono and Silvina Noemí Valdez

    BMC Biotechnology 2016 16:84

    Published on: 24 November 2016

  17. Methodology article

    Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization

    Due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (DENV), chikungunya and zika virus, have developed into major public health challenges....

    Natalie Rose, Carlos Augusto Pinho-Nascimento, Alessia Ruggieri, Paola Favuzza, Marco Tamborrini, Hanna Roth, Marcia Terezinha Baroni de Moraes, Hugues Matile, Thomas Jänisch, Gerd Pluschke and Katharina Röltgen

    BMC Biotechnology 2016 16:83

    Published on: 22 November 2016

  18. Research article

    Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification

    Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their compo...

    Wanwitoo Wanmolee, Warasirin Sornlake, Nakul Rattanaphan, Surisa Suwannarangsee, Navadol Laosiripojana and Verawat Champreda

    BMC Biotechnology 2016 16:82

    Published on: 21 November 2016

  19. Research article

    Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk

    Tobacco stalk is one kind of abundant crop residues in China. The high lignification of tobacco stalk increases its reusing cost and the existing of nicotine will cause serious pollution. The biodegradation of...

    Yulong Su, He Xian, Sujuan Shi, Chengsheng Zhang, S. M. Nuruzzaman Manik, Jingjing Mao, Ge Zhang, Weihong Liao, Qian Wang and Haobao Liu

    BMC Biotechnology 2016 16:81

    Published on: 21 November 2016

  20. Research article

    Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases

    Hexuronic acids such as D-galacturonic acid and D-glucuronic acid can be utilized via different pathways within the metabolism of microorganisms. One representative, the oxidative pathway, generates α-keto-glu...

    André Pick, Barbara Beer, Risa Hemmi, Rena Momma, Jochen Schmid, Kenji Miyamoto and Volker Sieber

    BMC Biotechnology 2016 16:80

    Published on: 17 November 2016

  21. Research article

    A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum

    Pyruvate kinase (Pyk) catalyzes the generation of pyruvate and ATP in glycolysis and functions as a key switch in the regulation of carbon flux distribution. Both the substrates and products of Pyk are involve...

    Xin Chai, Xiuling Shang, Yu Zhang, Shuwen Liu, Yong Liang, Yun Zhang and Tingyi Wen

    BMC Biotechnology 2016 16:79

    Published on: 16 November 2016

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