Microbial biotechnology

Section edited by Peter J Punt & Dennis Claessen

This section will consider studies that focus on the genetic manipulation and synthetic/systems biology of bacteria, fungi, yeasts and algae in the context of bioproduction, fermentation, metabolic engineering, biocatalysis, bioremediation and biodegradation.

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  1. Research article

    Improved n-butanol production via co-expression of membrane-targeted tilapia metallothionein and the clostridial metabolic pathway in Escherichia coli

    N-Butanol has favorable characteristics for use as either an alternative fuel or platform chemical. Bio-based n-butanol production using microbes is an emerging technology that requires further development. Al...

    Wei-Chih Chin, Kuo-Hsing Lin, Chun-Chi Liu, Kenji Tsuge and Chieh-Chen Huang

    BMC Biotechnology 2017 17:36

    Published on: 11 April 2017

  2. Methodology article

    A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends

    Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction s...

    Fanli Zeng, Zhimin Hao, Pan Li, Yanan Meng, Jingao Dong and Yibin Lin

    BMC Biotechnology 2017 17:32

    Published on: 17 March 2017

  3. Research article

    An engineered food-grade Lactococcus lactis strain for production and delivery of heat-labile enterotoxin B subunit to mucosal sites

    Recent researches have been focusing on mucosal immune adjuvants, which play the key roles in mucosal immunization and have become the limitation for non-injected vaccine development. Escherichia coli heat-labile...

    Nan Sun, Rongguang Zhang, Guangcai Duan, Xiaoyan Peng, Chen Wang, Qingtang Fan, Shuaiyin Chen and Yuanlin Xi

    BMC Biotechnology 2017 17:25

    Published on: 6 March 2017

  4. Research article

    Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol

    Manganese peroxidase (MnP) of white rot basidiomycetes, an extracellular heme enzyme, is part of a peroxidase superfamily that is capable of degrading the different phenolic compounds. Ganoderma, a white rot basi...

    Hui Xu, Meng-Yuan Guo, Yan-Hua Gao, Xiao-Hui Bai and Xuan-Wei Zhou

    BMC Biotechnology 2017 17:19

    Published on: 23 February 2017

  5. Research article

    Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

    poly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-sca...

    Jun Xia, Jiaxing Xu, Xiaoyan Liu, Jiming Xu, Xingfeng Wang and Xiangqian Li

    BMC Biotechnology 2017 17:20

    Published on: 23 February 2017

  6. Research article

    Penicillin production in industrial strain Penicillium chrysogenum P2niaD18 is not dependent on the copy number of biosynthesis genes

    Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many pr...

    Sandra Ziemons, Katerina Koutsantas, Kordula Becker, Tim Dahlmann and Ulrich Kück

    BMC Biotechnology 2017 17:16

    Published on: 16 February 2017

  7. Research article

    The antibacterial activity and mechanism of ginkgolic acid C15:1

    The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains.

    Zhebin Hua, Caie Wu, Gongjian Fan, Zhenxing Tang and Fuliang Cao

    BMC Biotechnology 2017 17:5

    Published on: 14 January 2017

  8. Research article

    Production of human pro-relaxin H2 in the yeast Pichia pastoris

    Initially known as the reproductive hormone, relaxin was shown to possess other therapeutically useful properties that include extracellular matrix remodeling, anti-inflammatory, anti-ischemic and angiogenic e...

    D. Cimini, K. Della Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. V. A. Pirozzi, F. Ferrara, R. Formisano, M. De Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone and C. Schiraldi

    BMC Biotechnology 2017 17:4

    Published on: 14 January 2017

  9. Research article

    Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk

    Tobacco stalk is one kind of abundant crop residues in China. The high lignification of tobacco stalk increases its reusing cost and the existing of nicotine will cause serious pollution. The biodegradation of...

    Yulong Su, He Xian, Sujuan Shi, Chengsheng Zhang, S. M. Nuruzzaman Manik, Jingjing Mao, Ge Zhang, Weihong Liao, Qian Wang and Haobao Liu

    BMC Biotechnology 2016 16:81

    Published on: 21 November 2016

  10. Research article

    A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum

    Pyruvate kinase (Pyk) catalyzes the generation of pyruvate and ATP in glycolysis and functions as a key switch in the regulation of carbon flux distribution. Both the substrates and products of Pyk are involve...

    Xin Chai, Xiuling Shang, Yu Zhang, Shuwen Liu, Yong Liang, Yun Zhang and Tingyi Wen

    BMC Biotechnology 2016 16:79

    Published on: 16 November 2016

  11. Methodology article

    Sequential and counter-selectable cassettes for fission yeast

    Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of i...

    Hanna Amelina, Vera Moiseeva, Laura Catharine Collopy, Siân Rosanna Pearson, Christine Anne Armstrong and Kazunori Tomita

    BMC Biotechnology 2016 16:76

    Published on: 8 November 2016

  12. Methodology article

    New tools to convert bacterial artificial chromosomes to a self-excising design and their application to a herpes simplex virus type 1 infectious clone

    Infectious clones are fundamental tools for the study of many viruses, allowing for efficient mutagenesis and reproducible production of genetically-defined strains. For the large dsDNA genomes of the herpesvi...

    Alexsia L. Richards, Patricia J. Sollars and Gregory A. Smith

    BMC Biotechnology 2016 16:64

    Published on: 31 August 2016

  13. Research article

    High-specificity synthesis of novel monomers by remodeled alcohol hydroxylase

    Diols are important monomers for the production of plastics and polyurethanes, which are widely used in our daily life. The medium-chain diols with one hydroxyl group at its subterminal end are able to confer ...

    Yanning Zheng, Lingling Li, Qiang Liu, Haibo Zhang, Yujin Cao, Mo Xian and Huizhou Liu

    BMC Biotechnology 2016 16:61

    Published on: 24 August 2016

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