Retraction: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10

  • Ondrej Kaplan1,

    Affiliated with

    • Karel Bezouska1, 2,

      Affiliated with

      • Ondrej Plihal1,

        Affiliated with

        • Rudiger Ettrich3,

          Affiliated with

          • Natallia Kulik3,

            Affiliated with

            • Ondrej Vanek1, 2,

              Affiliated with

              • Daniel Kavan1, 2,

                Affiliated with

                • Oldrich Benada1,

                  Affiliated with

                  • Anna Malandra1, 4,

                    Affiliated with

                    • Ondrej Sveda1,

                      Affiliated with

                      • Alicja B Vesela1,

                        Affiliated with

                        • Anna Rinagelova1,

                          Affiliated with

                          • Kristyna Slamova1,

                            Affiliated with

                            • Maria Cantarella4,

                              Affiliated with

                              • Jurgen Felsberg1,

                                Affiliated with

                                • Jarmila Duskova5,

                                  Affiliated with

                                  • Jan Dohnalek5,

                                    Affiliated with

                                    • Michael Kotik1,

                                      Affiliated with

                                      • Vladimir Kren1 and

                                        Affiliated with

                                        • Ludmila Martinkova1Email author

                                          Affiliated with

                                          BMC Biotechnology201313:57

                                          DOI: 10.1186/1472-6750-13-57

                                          Received: 12 July 2013

                                          Accepted: 12 July 2013

                                          Published: 20 July 2013

                                          Retraction

                                          The authors have retracted this article [1] due to the ethical misconduct of Karel Bezouska and misappropriation of data which fundamentally changes the main findings of the article. Specifically, the upper amino acid sequence in Figure 1 (by KB) in BMC Biotechnol 11:2 was incorrect, therefore Nit-ANigWT and Nit-ANigRec (nitrilase expressed in E. coli harbouring a nit gene from A. niger K10) were not variants of the same enzyme as hypothesized in the original article. The different biochemical properties of Nit-ANigWT and Nit-ANigRec discussed in the paper were caused by a significant difference in the primary structures of these enzymes.

                                          Authors’ Affiliations

                                          (1)
                                          Institute of Microbiology, Academy of Sciences of the Czech Republic
                                          (2)
                                          Department of Biochemistry, Faculty of Science, Charles University in Prague
                                          (3)
                                          Centre of Biocatalysis and Biotransformation, Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic
                                          (4)
                                          Department of Chemistry, Chemical Engineering and Materials, University of L’Aquila
                                          (5)
                                          Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic

                                          References

                                          1. Kaplan O, Bezouska K, Plihal O, Ettrich R, Kulik N, Vanek O, Kavan D, Benada O, Malandra A, Sveda O, Vesela AB, Rinagelova A, Slamova K, Cantarella M, Felsberg J, Duskova J, Dohnalek J, Kotik M, Kren V, Martinkova L: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10. BMC Biotechnol 2011, 11:2.View Article

                                          Copyright

                                          © Kaplan et al.; licensee BioMed Central Ltd. 2013

                                          This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                                          Advertisement