Erratum to: BTI-Tnao38, a new cell line derived from Trichoplusia ni, is permissive for AcMNPV infection and produces high levels of recombinant proteins
© Hashimoto et al; licensee BioMed Central Ltd. 2012
Received: 22 March 2012
Accepted: 24 April 2012
Published: 24 April 2012
The original article was published in BMC Biotechnology 2010 10:50
After publication we discovered an error in the identification of the origin of the cell line reported in our article in BMC Biotechnology (2010, 10:50), entitled "Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins". Upon analysis of primary A. odorata cultures, we found that they were contaminated with cells of Trichoplusia ni origin. The origin of the Ao38 cell line was determined as T. ni using three marker genes and the Ao38 cell line was renamed BTI-Tnao38. References to the origin of the cell line as Ascalapha odorata should be replaced with "a cell line of Trichoplusia ni origin". The absence of TNCL virus detection in the BTI-Tnao38 (Ao38) cell line was confirmed using a highly sensitive RT-PCR protocol capable of detecting TNCL virus RNA at approximately 0.018 copies/cell. Because of these observations, we have revised the title of the original article to "Correction: BTI-Tnao38, a new cell line derived from Trichoplusia ni, is permissive for AcMNPV infection and produces high levels of recombinant proteins" and two additional authors were added to reflect their contributions to the analysis of this cell line.
After publication of this work , we discovered an error in the identification of the origin of the cell line reported in this study. In this study, we described the isolation and detailed characterization of a cell line that produces high levels of recombinant proteins. In addition, we were unable to detect the presence of the Trichoplusia ni cell line virus (TNCLV), an alphanodavirus that was previously reported in a T. ni derived cell line . We reported that the Ao38 cell line was derived from a culture of primary cells of Ascalapha odorata. In the course of our subsequent characterization, we discovered that the source of the Ao38 cell line was not A. odorata as reported, but was instead T. ni. A careful analysis of archived intermediate stages in the production of the cell line indicated that the line resulted from primary A. odorata cultures that were contaminated with T. ni cells. While the source of the contamination is not known with certainty, further analysis suggests that Ao38 cells are likely a clonal derivative of the High Five (Tn-5B1-4) cell line. While we stand behind the analysis of the characteristics of this cell line, we are providing a correction to the above study, and we sincerely apologize for any problems or difficulties caused by our error in identification of the origin of this line. Below, we provide representative data establishing the identity of this line as a T. ni derived cell line. We also reexamined the cell line for the presence of TNCLV under conditions of defined sensitivity, and provide additional data confirming the absence of detection of the TNCLV alphanodavirus from this line.
To reflect the significant contributions of scientists involved in the extended analysis of the origin of the Ao38 cell line, and the analysis of TNCLV RNA detection and sensitivity, Shiying Zhang and Yun-Ru Chen were included as authors of this study.
The authors are grateful to Ping Wang for providing data and expertise on the analysis of cadherin gene sequences.
- Hashimoto Y, Zhang S, Blissard GW: Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins. BMC Biotechnol. 2010, 10 (1): 50-10.1186/1472-6750-10-50.View ArticleGoogle Scholar
- Li T-C, Scotti PD, Miyamura T, Takeda N: Latent Infection of a new Alphanodavirus in an insect cell line. J Virol. 2007, 81 (20): 10890-10896. 10.1128/JVI.00807-07.View ArticleGoogle Scholar
- Ratnasingham S, Hebert PDN: BOLD: The Barcode of Life Data System. Molecular Ecology Notes. 2007, doi: 10.1111/j.1471-8286.2006.01678.x, [http://www.barcodinglife.org]Google Scholar
- Hebert PD, Dewaard JR, Landry JF: DNA barcodes for 1/1000 of the animal kingdom. Biol Lett. 2010, 6 (3): 359-362. 10.1098/rsbl.2009.0848.View ArticleGoogle Scholar
- Hebert PD, Cywinska A, Ball SL, de Waard JR: Biological identifications through DNA barcodes. Proc Biol Sci/The Royal Soc. 2003, 270 (1512): 313-321. 10.1098/rspb.2002.2218.View ArticleGoogle Scholar
- Nieto Feliner G, Rossello JA: Better the devil you know? Guidelines for insightful utilization of nrDNA ITS in species-level evolutionary studies in plants. Mol Phylogenet Evol. 2007, 44 (2): 911-919. 10.1016/j.ympev.2007.01.013.View ArticleGoogle Scholar
- YEH S-C, LEE S-T, WU C-Y, WANG C-H: A cell line (NTU-MV) established from Maruca vitrata (Lepidoptera: Pyralidae): Characterization, viral susceptibility, and polyhedra production. J Invertebr Pathol. 2007, 96 (2): 138-146. 10.1016/j.jip.2007.04.004.View ArticleGoogle Scholar
- Wu CY, Lin HF, Wang CH, Lo CF: Identification of insect cell lines and cell-line cross-contaminations by nuclear ribosomal ITS sequences. J Appl Entomology. 2011, 135 (8): 601-610. 10.1111/j.1439-0418.2010.01574.x.View ArticleGoogle Scholar
- Zhang X: Sequence variation in cadherin alleles from the cabbage looper, Trichoplusia ni. 2007, M S. Thesis. Cornell UniversityGoogle Scholar
- Tiewsiri K, Wang P: Differential alteration of two aminopeptidases N associated with resistance to Bacillus thuringiensis toxin Cry1Ac in cabbage looper. Proc Natl Acad Sci USA. 2011, 108 (34): 14037-14042. 10.1073/pnas.1102555108.View ArticleGoogle Scholar
- Shan M, Zhang SY, Jiang L, Ma M, Li GX: Susceptibility to AcMNPV and expression of recombinant proteins by a novel cell clone derived from a Trichoplusia ni QAU-BTI-Tn9-4s cell line. Virol Sin. 2011, 26 (5): 297-305. 10.1007/s12250-011-3201-1.View ArticleGoogle Scholar
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R: DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994, 3 (5): 294-299.Google Scholar
- Lukhtanov VA, Sourakov A, Zakharov EV, Hebert PD: DNA barcoding Central Asian butterflies: increasing geographical dimension does not significantly reduce the success of species identification. Mol Ecol Resour. 2009, 9 (5): 1302-1310. 10.1111/j.1755-0998.2009.02577.x.View ArticleGoogle Scholar
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